Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)

Sharma, Sanjeev; Singh, Balwinder; Nagpal, Avinash; S. Virk, G.; A. Zaidi, A.

Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)

Sanjeev Sharma^{*, 1}, Balwinder Singh², Avinash Nagpal³, G. S. Virk³, A. A. Zaidi⁴

¹ Lyallpur Khalsa College, Jalandhar-144001, India

² Khalsa College, Amritsar-143001, India

³ Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India

⁴ Molecular Plant Virology Laboratory, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur 176061 (H.P.), India

Article Information

Identifiers and Pagination:

Year: 2009
Volume: 2
First Page: 27
Last Page: 31
Publisher ID: TOBIOJ-2-27
DOI: 10.2174/1874196700902010027

Article History:

Received Date: 11/11/2008
Revision Received Date: 12/12/2009
Acceptance Date: 27/01/2009
Electronic publication date: 01/4/2009
Collection year: 2009

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© 2009 Singh et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

^* Address correspondence to this author at the Department of Botany, Lyallpur Khalsa College, Jalandhar-144001, India; E-mail: sanbot@rediffmail.com

Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour 􀀁 C. deliciosa Tenora). This paper reports the various methods viz. Bioassay, ELISA and RT-PCR for indexing of ICRSV. Bioassay was performed on Chenopodium amaranticolor, Cucumis sativus, Nicotiana glutinosa, N. tabacum, Petunia hybrida and Phaseolus vulgaris. However necrotic local lesions were observed only in case of Chenopodium amaranticolor and Phaseolus vulgaris. Infected trees were also found positive by indirect ELISA. RT-PCR of the infected plants showed an amplification of 539 bp fragment corresponding to coat protein gene and gene for nucleic acid binding protein.

Keywords: Kinnow, ICRSV, Bioassay, ELISA, RT-PCR.

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RESEARCH ARTICLE

Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)

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