RESEARCH ARTICLE
Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)
Sanjeev Sharma*, 1, Balwinder Singh2, Avinash Nagpal3, G. S. Virk3, A. A. Zaidi4
Article Information
Identifiers and Pagination:
Year: 2009Volume: 2
First Page: 27
Last Page: 31
Publisher Id: TOBIOJ-2-27
DOI: 10.2174/1874196700902010027
Article History:
Received Date: 11/11/2008Revision Received Date: 12/12/2009
Acceptance Date: 27/01/2009
Electronic publication date: 01/4/2009
Collection year: 2009
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour C. deliciosa Tenora). This paper reports the various methods viz. Bioassay, ELISA and RT-PCR for indexing of ICRSV. Bioassay was performed on Chenopodium amaranticolor, Cucumis sativus, Nicotiana glutinosa, N. tabacum, Petunia hybrida and Phaseolus vulgaris. However necrotic local lesions were observed only in case of Chenopodium amaranticolor and Phaseolus vulgaris. Infected trees were also found positive by indirect ELISA. RT-PCR of the infected plants showed an amplification of 539 bp fragment corresponding to coat protein gene and gene for nucleic acid binding protein.