RESEARCH ARTICLE


Optimization of Pectate Lyase Production from Paenibacillus polymyxa N10 using Response Surface Methodology



Molnapat Songpim1, Pilanee Vaithanomsat*, 1, Sawitri Chuntranuluck2
1 Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok, 10900, Thailand
2 Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50, Chatuchak, Bangkok, 10900, Thailand


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 236
Abstract HTML Views: 482
PDF Downloads: 190
Total Views/Downloads: 908
Unique Statistics:

Full-Text HTML Views: 183
Abstract HTML Views: 370
PDF Downloads: 135
Total Views/Downloads: 688



© 2010 Songpim et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok, 10900, Thailand; Tel./Fax. 66-2942- 8599; E-mail: aappln@ku.ac.th, p_vaithanomsat@yahoo.com


Abstract

The parameters affecting the production of pectate lyase from P. polymyxa N10 were studied using the response surface methodology agitation rate (X1, 100-300 rpm), temperature (X2, 25-45 °C) and pH (X3, 5.5-9.5). The most significant factors influencing enzyme production were temperature and pH. The second order polynomial regression model obtained was fitted and found adequate, with an R2 of 0.9600 (p < 0.001). A maximum pectate lyase activity of 84.5 U/ml was attained in 72 h of cultivation at agitation rate 200 rpm, temperature 35 °C and pH 8. Optimizations of agitation rate and aeration on pectate lyase production were also carried out in a 5-l stirred-tank bioreactor. The aeration rate was varied in the range of 0.5-2 vvm at agitation rate of 200 rpm (temperature 35°C and initial pH 8). At agitation rate of 200 rpm, the shear force was high and then decreased the pectate lyase activity due to its negative effect on the enzyme structure. A maximum pectate lyase activity of 110.42 U/ml in the bioreactor was close to that obtained from the shake flask fermentation study.

Keywords: Pectate lyase, Paenibacillus polymyxa, response surface methodology.